Web supplement for the paper "Differential Gene Expression Profiling of Adult Murine Hematopoietic Stem Cells" by:
In-Kyung Park, Yaqin He, Fangming Lin, Ole D. Laerum, Qiang Tian, Roger Bumgarner, Christopher A. Klug, Kaijun Li, Christian Kuhr,Michelle J. Doyle, Tao Xie, Michel Schummer, Yu Sun, Adam Goldsmith, Michael F. Clarke, Irving L. Weissman, Leroy Hood, and Linheng Li


Introduction:

Hematopoietic stem cells (HSCs) have self-renewal capacity and multilineage developmental potentials. The molecular mechanisms that control the self-renewal of HSCs are still largely unknown. Here, we describe a systematic approach using bioinformatics and array hybridization techniques to analyze gene expression profiles in HSCs. To enrich mRNAs predominantly expressed in uncommitted cell lineages, 54,000 cDNA clones generated from a highly enriched population of HSCs and a mixed population of stem and early multipotent progenitor (MPP) cells were arrayed on nylon membranes (macroarray or high-density array), and subtracted with cDNA probes derived from mature lineage cells including spleen, thymus, and bone marrow. Five thousand cDNA clones with very low hybridization signals were selected for sequencing and further analysis using microarrays on glass slides. Two populations of cells, HSCs and MPP, were compared for differential gene expression using microarray analysis. HSCs have the ability to self-renew, while MPP have lost the capacity for self-renewal. A large number of genes that were differentially expressed by enriched populations of HSCs and MPP were identified. These included transcription factors, signaling molecules, and previously unknown genes.


Method:


Query:         Click here to get full list of all the sequence. (Please contact Dr. Qiang Tian for the access code to open it.)

By ID of the clone:

By txt (keyword) in the description of five best blast hits:

Example: kinase

By txt (sequence) in database :  



Contact info:

For questions related to the paper:
Linheng Li, Ph.D.
Assistant Stowers Scientist
Stowers Institute For Medical Research
lil@stowers-institute.org
816-926-4081

Or:
Qiang Tian, M.D., Ph.D.
Stem Cell Group
The Institute For Systems Biology
qtian@systemsbiology.org
206-732-1308

 

The web-database was built by:
Tao Xie, Ph.D
The Institute For Systems Biology
txie@systemsbiology.org
206-732-1396

Address information of all authors, please click here.

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