Web supplement for the paper "Differential Gene Expression Profiling of Adult Murine Hematopoietic Stem Cells" by:
Hematopoietic stem cells (HSCs) have self-renewal capacity and multilineage developmental potentials. The molecular mechanisms that control the self-renewal of HSCs are still largely unknown. Here, we describe a systematic approach using bioinformatics and array hybridization techniques to analyze gene expression profiles in HSCs. To enrich mRNAs predominantly expressed in uncommitted cell lineages, 54,000 cDNA clones generated from a highly enriched population of HSCs and a mixed population of stem and early multipotent progenitor (MPP) cells were arrayed on nylon membranes (macroarray or high-density array), and subtracted with cDNA probes derived from mature lineage cells including spleen, thymus, and bone marrow. Five thousand cDNA clones with very low hybridization signals were selected for sequencing and further analysis using microarrays on glass slides. Two populations of cells, HSCs and MPP, were compared for differential gene expression using microarray analysis. HSCs have the ability to self-renew, while MPP have lost the capacity for self-renewal. A large number of genes that were differentially expressed by enriched populations of HSCs and MPP were identified. These included transcription factors, signaling molecules, and previously unknown genes.
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